Bacteriostatic Water for Injection (BWFI) is a sterile, non-pyrogenic solution of water that contains 0.9% benzyl alcohol (9 mg/mL) as a preservative. It is used to dilute or dissolve medications for injection and is distinct from sterile water in that it inhibits bacterial growth, allowing for multiple uses from the same vial within a specified time.
Key Components
Water for Injection (WFI): Sterile, purified water that meets pharmacopeial standards.
Benzyl Alcohol (0.9%): A bacteriostatic agent that prevents microbial contamination after opening.
Primary Uses
1. Peptide Reconstitution
Commonly used to reconstitute lyophilized peptides (e.g., BPC-157, GHK-Cu, PT-141) before subcutaneous injection.
Preserves peptide stability for up to 28 days if refrigerated.
2. Injectable Medications
Used to dilute drugs like hormones (HGH, HCG), antibiotics, or other IV/IM injectable medications.
3. Multi-dose Applications
Unlike sterile water (single-use), bacteriostatic water allows for multiple withdrawals from the same vial without contamination, making it cost-effective and practical for repeated dosing.
How to Use Bacteriostatic Water
Reconstituting Peptides (Example)
Prepare materials: Alcohol wipes, syringe, vial of peptide, bacteriostatic water.
Clean vial tops with alcohol.
Draw desired amount of bacteriostatic water into a syringe (e.g., 1 mL).
Inject water slowly into the peptide vial (against the side of the vial to avoid foam).
Gently swirl until fully dissolved. Do not shake.
Storage Instructions
Store reconstituted peptides in the refrigerator (2–8°C / 36–46°F).
Once opened, a vial of bacteriostatic water should be used within 28 days.
Advantages
Feature
Bacteriostatic Water
Sterile Water
Contains Preservative
Yes (benzyl alcohol)
No
Multi-use (within 28 days)
Yes
No (single use only)
Shelf Life After Opening
~28 days
Use immediately
Use in Peptide Reconstitution
Ideal
Acceptable for one-time use
Cautions and Considerations
Not for use in neonates: Benzyl alcohol is associated with “gasping syndrome” in newborns.
Do not use IV in large volumes: The benzyl alcohol content makes it unsuitable for large-volume intravenous infusions.
Use aseptic technique: Always wipe vials and use sterile equipment to avoid contamination.
Storage and Shelf Life
Unopened Vial: Typically has a 2–3 year shelf life when stored at room temperature.
After Opening: Must be used within 28 days due to potential bacterial contamination over time.
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